Researchers develop portable biosensor that rapidly detects avian influenza
By admin | March 13, 2010
http://dailyheadlines.uark.edu/9896.htm
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FOR RELEASE: Thursday, January 25, 2007
Rapid Response to Avian Flu Threat
Researchers develop portable biosensor that rapidly detects avian influenza.
FAYETTEVILLE, Ark. - An interdisciplinary team of researchers led by Yanbin Li, professor of biological engineering in the University of Arkansas Division of Agriculture, has developed a portable biosensor for in-field, rapid screening of avian influenza virus. The inexpensive device specifically and sensitively detects the avian influenza strain H5N1 from poultry cloacal or tracheal swab samples in less than 30 minutes and could help health officials coordinate a rapid response for the eradication, quarantine and vaccination of animals. [....] Based on Li's previous research on impedance biosensors to detect Salmonella and E. coli, the new system uses magnetic bio-nanobeads, a specially designed microfluidic biochip and red blood cell complexes to detect the virus. Detection follows a step-by-step process. First, researchers coat the magnetic nanobeads with specific antibodies to separate and concentrate the target virus within a poultry swab sample. Acting as bio-labels, red blood cells are then mixed with the captured virus to form a complex, which is filtered by a specially designed, micro-fluidic biochip. The biochip then delivers the complex to a microelectrode array for measurement. Researchers detect the specific virus by correlating the change in impedance of the complex under high-frequency and alternating currents to the concentration of avian influenza virus in an original sample.
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